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Research Highlights
 
Cryo-electron tomography reveals the structure and dynamics of an alphacoronavirus spike protein
  Huang CY, Draczkowski P, Wang YS, Chang CY, Chien YC, Cheng YH, Wu YM, Wang CH, Chang YC, Chang YC, Yang TJ, Tsai YX, Khoo KH, Chang HW, Hsu STD*
Mono-GlcNAc-decorated spike vaccine is highly effective against COVID-19 variants
  Huang HY, Liao HY, Chen XR, Wang SW, Cheng CW, Mahmud MSA, Liu YM, Mohapatra A, Chen TH, Lo JM, Wu YM, Ma HH, Chang YH, Tsai HY, Chou YC, Hsueh YP, Tsai CY, Huang PY Chang SY, Chao TL, Kao HC, Tsai YM, Chen YH, Wu CY, Jan JT, Cheng TJR, Lin KI*, Ma C*, Wong CH*
Therapeutic Antibody Cocktail for Prevention and Treatment of COVID-19
  Su SC, Yang TJ, Yu PY, Liang KH, Chen WY, Yang CW, Lin HT, Wang MJ, Lu RM, Tso HC, Chung MJ, Hsieh TY, Chang YL, Lin SC, Hsu FY, Ke FY, Wu YH, Hwang YC, Liu IJ, Liang JJ, Liao CC, Ko HY, Sun CP, Wu PY, Jan JT, Chang YC, Lin YL*, Tao MH*, Hsu STD*, Wu HC*
Molecular insights into the increased transmissibility and immunity evasion of the SARS-CoV-2 Alpha variant
  Yang TJ, Yu PY, Chang YC, Liang KH, Tso HC, Ho MR, Chen WY, Lin HT, Wu HC, Hsu STD*
Copper Centers in the Cryo-EM Structure of Particulate Methane Monooxygenase Reveal the Catalytic Machinery of Methane Oxidation
  Chang WH*, Lin HH, Tsai IK, Huang SH, Chung SC, Tu IP, Yu SSF*, Chan SI*
Direct Visualization of a 26 kDa Protein by Cryo-Electron Microscopy Aided by a Small Scaffold Protein
  Chiu YH, Ko KT, Yang TJ, Wu KP, Ho MR, Draczkowski P*, Hsu STD*
Perfection in the imperfection – molecular mechanism for genetic diversity
  Luo SC, Yeh HY, Lan WH, Wu YM, Yang CH, Chang HY, Su GC, Lee CY, Wu WJ, Li HW, Ho MC*, Chi P*, Tsai MD*
FRIL Can Grab the Thorny Crown to Fight Off COVID-19
  Liu YM, Mahmud Md. SA, Chen XR, Chen TH, Liao KS, Lo JM, Wu YM, Ho MC, Wu CY, Wong CH, Jan JT, Ma C
Through the looking glass of cryoEM, unveiling the unique molecular structure and camouflaging sugar coating of feline coronavirus surface spike protein
  Yang TJ, Chang YC, Ko TP, Draczkowski P, Chien YC, Chang YC, Wu KP, Khoo KH, Chang HW*, Hsu STD*
Sharp Tools Make Good Work: The Early Success of ASCEM
  Chen CY, Chang YC, Lin BL, Lin KF, Huang CH, Hsieh DL, Ko TP, Tsai MD
New Research Highlight on the Lon Protease
 
1. Chien-Chu Lin, Shih-Chieh Su, Ming-Yuan Su, Pi-Hui Liang, Chia-Cheng Feng, Shih-Hsiung Wu, Chung-I Chang
2.
 
Shih-Chieh Su, Chien-Chu Lin, Hui-Chung Tai, Mu-Yueh Chang, Meng-Ru Ho, C. Satheesan Babu, Jiahn-Haur Liao, Shih-Hsiung Wu, Yuan-Chih Chang, Carmay Lim, Chung-I Chang
 
Latest Publication
 
In situ structure and dynamics of an alphacoronavirus spike protein by cryo-ET and cryo-EM.
  Huang CY, Draczkowski P, Wang YS, Chang CY, Chien YC, Cheng YH, Wu YM, Wang CH, Chang YC, Chang YC, Yang TJ, Tsai YX, Khoo KH, Chang HW, Hsu STD*
Nature Communications (2022)
2.2 Å Cryo-EM Tetra-Protofilament Structure of the Hamster Prion 108–144 Fibril Reveals an Ordered Water Channel in the Center.
  Chen EHL, Kao HW, Lee CH, Huang JYC, Wu KP*, Chen RPY*
J. Am. Chem. Soc. (2022)
Structural basis for the helical filament formation of Escherichia coli glutamine synthetase.
  Huang PC, Chen SK, Chiang WH, Ho MR, Wu KP*
Protein Science (2022)
Vaccination with SARS-CoV-2 spike protein lacking glycan shields elicits enhanced protective responses in animal models.
  Huang HY, Liao HY, Chen XR, Wang SW, Cheng CW, Mahmud MSA, Liu YM, Mohapatra A, Chen TH, Lo JM, Wu YM, Ma HH, Chang YH, Tsai HY, Chou YC, Hsueh YP, Tsai CY, Huang PY Chang SY, Chao TL, Kao HC, Tsai YM, Chen YH, Wu CY, Jan JT, Cheng TJR, Lin KI*, Ma C*, Wong CH*
Science Translational Medicine (2022)
A 10-nm-sized multicompartment cuboctahedron and its 2D hierarchical arrays observed by cryo-EM.
  He LP, Hsu HK, Li LJ, Lin LT, Tu TH, Ong TG, Liou GG*, Chan YT*
Chem (2022)
Distinct shifts in site-specific glycosylation pattern of SARS-CoV-2 spike proteins associated with arising mutations in the D614G and Alpha variants.
  Kuo CW, Yang TJ, Chien YC, Yu PY, Hsu STD*, Khoo KH*
Glycobiology (2022)
Liposomal PHD2 Inhibitors and the Enhanced Efficacy in Stabilizing HIF-1α.
  Jian CB, Yu XE, Gao HD, Chen HA, Jheng RH, Chen CY, Lee HM*
Nanomaterials (Basel) (2022)
Multifunctional carbonized nanogels to treat lethal acute hepatopancreatic necrosis disease.
  Yen SC, Mao JY, Lin HY, Huang HT, Harroun SG, Nain A, Chang HT, Lin HY, Chen LL, Huang CC, Lin HJ
J Nanobiotechnol (2021)
Catalytic machinery of methane oxidation in particulate methane monooxygenase (pMMO).
  Chan S I, Chang WH, Huang SH, Lin HH, Yu S SF
J Inorg Biochem (2021)
Processive cleavage of substrate at individual proteolytic active sites of the Lon protease complex.
  Li SS, Hsieh KY, Kuo CI, Su SC, Huang KF, Zhang KM, Chang CI
Science Advances (2021)
Structure-guided antibody cocktail for prevention and treatment of COVID-19.
  Su SC, Yang TJ, Yu PY, Liang KH, Chen WY, Yang CW, Lin HT, Wang MJ, Lu RM, Tso HC, Chung MJ, Hsieh TY, Chang YL, Lin SC, Hsu FY, Ke FY, Wu YH, Hwang YC, Liu IJ, Liang JJ, Liao CC, Ko HY, Sun CP, Wu PY, Jan JT, Chang YC, Lin YL*, Tao MH*, Hsu STD*, Wu HC*
PLOS Pathogens (2021)
Complete three-dimensional structures of the Lon protease translocating a protein substrate.
  Li SS, Hsieh KY, Kuo CI, Lee SH, Pintilie GD, Zhang KM, Chang CI
Science Advances (2021)
Molecular basis for ATPase-powered substrate translocation by the Lon AAA+ protease.
  Li SS, Hsieh KY, Su SC, Pintilie GD, Zhang KM, Chang CI
Journal of Biological Chemistry (2021)
D614G mutation in the SARS-CoV-2 spike protein enhances viral fitness by desensitizing it to temperature-dependent denaturation.
  Yang TJ, Yu PY, Chang YC, Hsu STD*
Journal of Biological Chemistry (2021)
Polyethylene Glycol Immunogenicity: Theoretical, Clinical, and Practical Aspects of Anti-Polyethylene Glycol Antibodies.
  Chen BM, Cheng TL, Roffler SR
ACS Nano (2021)
Effect of SARS-CoV-2 B.1.1.7 mutations on spike protein structure and function.
  Yang TJ, Yu PY, Chang YC, Liang KH, Tso HC, Ho MR, Chen WY, Lin HT, Wu HC, Hsu STD*
Nature Structural & Molecular Biology (2021)
Copper Centers in the Cryo-EM Structure of Particulate Methane Monooxygenase Reveal the Catalytic Machinery of Methane Oxidation.
  Chang WH*, Lin HH, Tsai IK, Huang SH, Chung SC, Tu IP, Yu SSF*, Chan SI*
J. Am. Chem. Soc. (2021)
Preparation of High-Temperature Sample Grids for Cryo-EM.
  Chang YC, Chen CY, Tsai MD*
Jove - Journal of Visualized Experiments (2021)
Sub-3 Å Cryo-EM Structures of Necrosis Virus Particles via the Use of Multipurpose TEM with Electron Counting Camera
  Wang CH, Chen DH, Huang SH, Wu YM, Chen YY, Hwu YK, Bushnell D, Kornberg R, Chang WH*
International Journal of Molecular Sciences (2021)
Robust induction of resident memory T cells by combinatorial nanoshells confers cross-strain sterilizing immunity against lethal influenza viruses.
  Lin PH, Liang CY, Yao BY, Chen HW, Pan CF, Wu LL, Lin YH, Hsu YS, Liu YH, Chen PJ, Hu CMJ, Yang HC
Methods & Clinical Development (2021)
Impact of Glycosylation on SARS-CoV-2 Infection and Broadly Protective Vaccine Design.
  Liao HY, Huang HY, Chen XR, Cheng CW, Wang SW, Mahmud MSA, Chen TH, Lo JM, Liu YM, Ma HH,Chang YH, Tsai CY, Huang PY, Chang SY, Chao TL, Kao HC, Tsai YM, Chen YH, Chen CY, Lee KC, Wu CY, Jan JT, Kuo-I Lin, Cheng TJR, Ma C, Wong CH
bioRxiv (2021)
Direct Visualization of a 26 kDa Protein by Cryo-Electron Microscopy Aided by a Small Scaffold Protein.
  Chiu YH, Ko KT, Yang TJ, Wu KP, Ho MR, Draczkowski P*, Hsu STD*
Biochemistry (2021)
Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures.
  Luo SC, Yeh HY, Lan WH, Wu YM, Yang CH, Chang HY, Su GC, Lee CY, Wu WJ, Li HW, Ho MC*, Chi P*, Tsai MD*
Nat Commun (2021)
Discrete Metal-Oxide Clusters with Organofunctionalization as High-Performance Anode Materials.
  Jadhav TS, Abbas SA, Liu YC, Wu WT, Lee GH, Chu CW, Chiang MH
ACS Appl. Energy Mater. (2021)
A carbohydrate-binding protein from the edible Lablab beans effectively blocks the infections of influenza viruses and SARS-CoV-2.
  Liu YM, Mahmud Md. SA, Chen XR, Chen TH, Liao KS, Lo JM, Wu YM, Ho MC, Wu CY, Wong CH, Jan JT, Ma C
Cell Reports (2020)
Premature Drug Release from Polyethylene Glycol (PEG)-Coated Liposomal Doxorubicin via Formation of the Membrane Attack Complex.
  Chen V, Chen BM, S YC, Chang YC, Cheng TL, Barenholz, Roffler SR
ACS Nano (2020)
XFEL coherent diffraction imaging for weakly scattering particles using heterodyne interference.
  Huang CF, Chang WH, Lee TK, Joti Y., Nishino Y., Kimura T., Suzuki A., Bessho Y., Lee TT, Chen MC, Yang SM, Hwu Y., Huang SH, Li PN, Chen P., Tseng YC, Ma C., Hsu TL., Wong CH, Tono K., Ishikawa T., Liang KS
AIP Advances (2020)
Cholesteryl α-D-glucoside 6-acyltransferase enhances the adhesion of Helicobacter pylori to gastric epithelium.
  Jan HM, Chen YC, Yang TC, Ong LL, Chang CC, Muthusamy S, Abera AB, Wu MS, Gervay-Hague J, Mong KKT, Lin CH
Communications Biology (2020)
Cryo-EM analysis of a feline coronavirus spike protein reveals a unique structure and camouflaging glycans.
  Yang TJ, Chang YC, Ko TP, Draczkowski P, Chien YC, Chang YC, Wu KP, Khoo KH, Chang HW, Hsu STD
PNAS (2020)
Temperature-Resolved Cryo-EM Uncovers Structural Bases of Temperature-Dependent Enzyme Functions.
  Chen CY, Chang YC, Lin BL, Huang CH, Tsai MD
J Am Chem Soc. (2019)
pH-Responsive PEG-Shedding and Targeting Peptide-Modified Nanoparticles for Dual-Delivery of Irinotecan and microRNA to Enhance Tumor-Specific Therapy.
  Juang V, Chang CH, Wang, CS, Wang HE, Lo YL
Small (2019)
Use of Cryo-EM to Uncover Structural Bases of pH Effect and Cofactor Bi-specificity of Ketol-acid Reductoisomerase.
  Chen CY, Chang YC, Lin BL, Lin KF, Huang CH, Hsieh DL, Ko TP, Tsai MD
J Am Chem Soc. (2019)
Egg-based influenza split virus vaccine with monoglycosylation induces cross-strain protection against influenza virus infections.
  Tseng YC, Wu CY, Liu ML, Chen TH, Chiang WL, Yu YH, Jan JT, Lin KI, Wong CH, Ma C
PNAS (2019)
3D mitochondrial ultrastructure of Drosophila indirect flight muscle revealed by serial-section electron tomography.
  Jiang YF, Lin HL, Fu CY
J Vis Exp. (2017)
Electron tomographic analysis reveals ultrastructural features of mitochondrial cristae architecture which reflect energetic state and aging.
  Jiang YF, Lin SS, Chen JM, Tsai HZ, Hsieh TS, Fu CY
Sci Rep. (2017)
Structural Basis for the Magnesium-Dependent Activation and Hexamerization of the Lon AAA+ Protease.
  Su SC, Lin CC, Tai HC, Chang MY, Ho MR, Babu CS, Liao JH, Wu SH, Chang YC, Lim C, Chang CI
Structure (2016)
EM structure of a helicase-loader complex depicting a 6:2 binding sub-stoichiometry from Geobacillus kaustophilus HTA426.
  Lin YC, Naveen V, Hsiao CD.
Biochem Biophys Res Commun (2016)
Structure of yeast Ape1 and its role in autophagic vesicle formation.
  Su MY, Peng WH, Ho MR, Su SC, Chang YC, Chen GC, Chang CI
Autophagy (2015)
 

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