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Research Highlights
 
Therapeutic Antibody Cocktail for Prevention and Treatment of COVID-19
  Su SC, Yang TJ, Yu PY, Liang KH, Chen WY, Yang CW, Lin HT, Wang MJ, Lu RM, Tso HC, Chung MJ, Hsieh TY, Chang YL, Lin SC, Hsu FY, Ke FY, Wu YH, Hwang YC, Liu IJ, Liang JJ, Liao CC, Ko HY, Sun CP, Wu PY, Jan JT, Chang YC, Lin YL*, Tao MH*, Hsu STD*, Wu HC*
Molecular insights into the increased transmissibility and immunity evasion of the SARS-CoV-2 Alpha variant
  Yang TJ, Yu PY, Chang YC, Liang KH, Tso HC, Ho MR, Chen WY, Lin HT, Wu HC, Hsu STD*
Copper Centers in the Cryo-EM Structure of Particulate Methane Monooxygenase Reveal the Catalytic Machinery of Methane Oxidation
  Chang WH*, Lin HH, Tsai IK, Huang SH, Chung SC, Tu IP, Yu SSF*, Chan SI*
Direct Visualization of a 26 kDa Protein by Cryo-Electron Microscopy Aided by a Small Scaffold Protein
  Chiu YH, Ko KT, Yang TJ, Wu KP, Ho MR, Draczkowski P*, Hsu STD*
Perfection in the imperfection – molecular mechanism for genetic diversity
  Luo SC, Yeh HY, Lan WH, Wu YM, Yang CH, Chang HY, Su GC, Lee CY, Wu WJ, Li HW, Ho MC*, Chi P*, Tsai MD*
FRIL Can Grab the Thorny Crown to Fight Off COVID-19
  Liu YM, Mahmud Md. SA, Chen XR, Chen TH, Liao KS, Lo JM, Wu YM, Ho MC, Wu CY, Wong CH, Jan JT, Ma C
Through the looking glass of cryoEM, unveiling the unique molecular structure and camouflaging sugar coating of feline coronavirus surface spike protein
  Yang TJ, Chang YC, Ko TP, Draczkowski P, Chien YC, Chang YC, Wu KP, Khoo KH, Chang HW*, Hsu STD*
Sharp Tools Make Good Work: The Early Success of ASCEM
  Chen CY, Chang YC, Lin BL, Lin KF, Huang CH, Hsieh DL, Ko TP, Tsai MD
New Research Highlight on the Lon Protease
 
1. Chien-Chu Lin, Shih-Chieh Su, Ming-Yuan Su, Pi-Hui Liang, Chia-Cheng Feng, Shih-Hsiung Wu, Chung-I Chang
2.
 
Shih-Chieh Su, Chien-Chu Lin, Hui-Chung Tai, Mu-Yueh Chang, Meng-Ru Ho, C. Satheesan Babu, Jiahn-Haur Liao, Shih-Hsiung Wu, Yuan-Chih Chang, Carmay Lim, Chung-I Chang
 
Latest Publication
 
Processive cleavage of substrate at individual proteolytic active sites of the Lon protease complex.
  Li SS, Hsieh KY, Kuo CI, Su SC, Huang KF, Zhang KM, Chang CI
Science Advances (2021)
Structure-guided antibody cocktail for prevention and treatment of COVID-19.
  Su SC, Yang TJ, Yu PY, Liang KH, Chen WY, Yang CW, Lin HT, Wang MJ, Lu RM, Tso HC, Chung MJ, Hsieh TY, Chang YL, Lin SC, Hsu FY, Ke FY, Wu YH, Hwang YC, Liu IJ, Liang JJ, Liao CC, Ko HY, Sun CP, Wu PY, Jan JT, Chang YC, Lin YL*, Tao MH*, Hsu STD*, Wu HC*
PLOS Pathogens (2021)
Complete three-dimensional structures of the Lon protease translocating a protein substrate.
  Li SS, Hsieh KY, Kuo CI, Lee SH, Pintilie GD, Zhang KM, Chang CI
Science Advances (2021)
D614G mutation in the SARS-CoV-2 spike protein enhances viral fitness by desensitizing it to temperature-dependent denaturation.
  Yang TJ, Yu PY, Chang YC, Hsu STD*
Journal of Biological Chemistry (2021)
Effect of SARS-CoV-2 B.1.1.7 mutations on spike protein structure and function.
  Yang TJ, Yu PY, Chang YC, Liang KH, Tso HC, Ho MR, Chen WY, Lin HT, Wu HC, Hsu STD*
Nature Structural & Molecular Biology (2021)
Copper Centers in the Cryo-EM Structure of Particulate Methane Monooxygenase Reveal the Catalytic Machinery of Methane Oxidation.
  Chang WH*, Lin HH, Tsai IK, Huang SH, Chung SC, Tu IP, Yu SSF*, Chan SI*
J. Am. Chem. Soc. (2021)
Preparation of High-Temperature Sample Grids for Cryo-EM.
  Chang YC, Chen CY, Tsai MD*
Jove - Journal of Visualized Experiments (2021)
Impact of Glycosylation on SARS-CoV-2 Infection and Broadly Protective Vaccine Design.
  Liao HY, Huang HY, Chen XR, Cheng CW, Wang SW, Mahmud MSA, Chen TH, Lo JM, Liu YM, Ma HH,Chang YH, Tsai CY, Huang PY, Chang SY, Chao TL, Kao HC, Tsai YM, Chen YH, Chen CY, Lee KC, Wu CY, Jan JT, Kuo-I Lin, Cheng TJR, Ma C, Wong CH
bioRxiv (2021)
Direct Visualization of a 26 kDa Protein by Cryo-Electron Microscopy Aided by a Small Scaffold Protein.
  Chiu YH, Ko KT, Yang TJ, Wu KP, Ho MR, Draczkowski P*, Hsu STD*
Biochemistry (2021)
Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures.
  Luo SC, Yeh HY, Lan WH, Wu YM, Yang CH, Chang HY, Su GC, Lee CY, Wu WJ, Li HW, Ho MC*, Chi P*, Tsai MD*
Nat Commun (2021)
A carbohydrate-binding protein from the edible Lablab beans effectively blocks the infections of influenza viruses and SARS-CoV-2.
  Liu YM, Mahmud Md. SA, Chen XR, Chen TH, Liao KS, Lo JM, Wu YM, Ho MC, Wu CY, Wong CH, Jan JT, Ma C
Cell Reports (2020)
Cryo-EM analysis of a feline coronavirus spike protein reveals a unique structure and camouflaging glycans.
  Yang TJ, Chang YC, Ko TP, Draczkowski P, Chien YC, Chang YC, Wu KP, Khoo KH, Chang HW, Hsu STD
PNAS (2020)
Temperature-Resolved Cryo-EM Uncovers Structural Bases of Temperature-Dependent Enzyme Functions.
  Chen CY, Chang YC, Lin BL, Huang CH, Tsai MD
J Am Chem Soc. (2019)
Use of Cryo-EM to Uncover Structural Bases of pH Effect and Cofactor Bi-specificity of Ketol-acid Reductoisomerase.
  Chen CY, Chang YC, Lin BL, Lin KF, Huang CH, Hsieh DL, Ko TP, Tsai MD
J Am Chem Soc. (2019)
3D mitochondrial ultrastructure of Drosophila indirect flight muscle revealed by serial-section electron tomography.
  Jiang YF, Lin HL, Fu CY
J Vis Exp. (2017)
Electron tomographic analysis reveals ultrastructural features of mitochondrial cristae architecture which reflect energetic state and aging.
  Jiang YF, Lin SS, Chen JM, Tsai HZ, Hsieh TS, Fu CY
Sci Rep. (2017)
Structural Basis for the Magnesium-Dependent Activation and Hexamerization of the Lon AAA+ Protease.
  Su SC, Lin CC, Tai HC, Chang MY, Ho MR, Babu CS, Liao JH, Wu SH, Chang YC, Lim C, Chang CI
Structure (2016)
EM structure of a helicase-loader complex depicting a 6:2 binding sub-stoichiometry from Geobacillus kaustophilus HTA426.
  Lin YC, Naveen V, Hsiao CD.
Biochem Biophys Res Commun (2016)
Structure of yeast Ape1 and its role in autophagic vesicle formation.
  Su MY, Peng WH, Ho MR, Su SC, Chang YC, Chen GC, Chang CI
Autophagy (2015)
 

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