About Access CryoSample/Data Training People Announcement Research Links Chinese
 Training

To facilitate new users to be familiar with Facility and related software, ASCEM will host seminars, training courses or workshop non-periodically.
 
Training Course
 
Course Announcement
 
(Training) 2019/10/14-2019/10/15 9:30-17:00 IBC R102 (Postponed to 2019/10/28-2019/10/29 10:00-17:00. 10/30 is open for 1-on-1 discussion.)
Dr. Jinhuan Chen from ThermoFisher Scientific “FAS training course - Relion Practical (advanced level, in Chinese)” One senior CryoEM user from each lab is recommended as a seed representative of this training course, and he/she is ASCEM CEM3/4 user as well as has experience with Relion data analysis. For register, please email to Ms. Hsin‐Hua Lin linhh@gate.sinica.edu.tw Registration Deadline: 2019.10.07 Monday.   Photo1   Photo2
(Training) 2019/02/18 10:00-15:00 IBC R303
Dr. Jinhuan Chen from Thermo Fisher Scientific “FAS training course – Relion (entry level, in Chinese)”
(Training) 2018/05/10-2018/05/11 A “Cryo-EM Advance Training Course”, limited to CEM2 users, is now open for registration. The deadline is at midnight (12:00) of May 04, 2018. Note: A PI signed course form (see attachment) is required on site. More   Form   Photo1   Photo2
(Training) Re-opened! The postponed course (2018/04/10-2018/04/13) is now re-scheduled to 2018/05/15-2018/05/18. The lecture is in Chinese. Looking forward to seeing you there on time and thank you for your patience.   Photo1   Photo2
(Training) Postponed! 2018/04/10-2018/04/13 “Cryo-EM Basic Training Course” is postponed because the instrument for the hands-on training (EM operation) has an unexpected problem and is under repairing and maintenance. The new dates will be announced once being decided. Please pardon us for the inconvenience caused.
(Training) 2018.04.10-2018.04.13 A “Cryo-EM Basic Training Course”, with a limit of 36 trainees, is now open for registration, click HERE! The deadline is at midnight (12:00) of April 02, 2018 or closed earlier when the register quota is full. More
(Workshop) 2018/03/21-2018/03/23 “CryoEM Workshop in 2018 International Symposium on Grid and Cloud (ISGC)”, welcome to join us! More
(Training) 2018/03/06-2018/03/07 A “Cryo-EM Basic Training Course” is now open for registration, click HERE! The deadline is at midnight (12:00) of February 26, 2018.More   Photo1   Photo2   Photo3
 
 
Seminar & Symposia
 
(Symposium) 2020/08/24-2020/08/27 “CryoEM Single Particle Analysis Workshop: what you need to know as a team leader”, for faculty or lab leaders only, limited to 20 attendees! More   Poster
(Seminar) 2020/07/07 14:00-15:30 IBC R209
Dr. Fu-Tong Liu from IBMS “What we are interested in visualizing by cryo-EM: intracellular glycan-galectin interaction”
(Seminar) 2020/06/23 14:00-15:30 IBC R209
Dr. Chung-Ju Wang from IPMB “Cryo-ET analysis of synaptonemal complex and its regulatory roles in meiotic recombination”
(Seminar) 2020/06/09 14:00-15:30 IBC R209
Dr. Lee-Jene Lai from NSRRC “Imaging 3D cells by Correlation of fluorescence microscopy with Soft X-ray Tomography”
(Seminar) 2020/05/26 14:00-15:30 IBC R209
Prof. Han-Yi Fu from Department of Biological Sciences, NSYSU “Elucidating the structural organization responsible for the photosystem II fluorescence quenching in red algae”
(Seminar) 2020/05/12 14:00-15:30 IBC Rm 209
Dr. Pei-Yu Wu from Dr. Ming-Daw Tsai Lab, IBC “Proposed Cryo-ET studies of DNA damage response complexes”
(Seminar) 2020/04/28 14:00-15:30 IBC Rm 209
Dr. Erh Min Lai from IPMB “Structure-function studies of a bacterial type IV secretion system”
(Seminar) 2020/04/14 14:00-15:30 IBC R209
Dr. Wen Chang from IMB “Using ECT to study virus membrane wrapping in cells”
(Seminar) 2020/03/17 14:00-15:30 IBC R303
Dr. Jen-Fei Chu, Postdoc from Dr. Che-Kun Shen’s Lab, IMB “High-resolution imaging studies of TDP-43 on pathogenic mRNP granules”
(Seminar) 2020/03/03 14:00-15:30 IBC R303
Dr. Chi-yu Fu from ICOB “Trial report for cryo-ET study of mammalian cells”
(Seminar) 2020/02/19 10:30-12:00 GRC Auditorium
Dr. Ming-Daw Tsai from IBC “New Horizon in Enzymology from Cryo-EM and X-ray Free-electron Laser (XFEL)”
(Seminar) 2020/02/18 14:00-15:30 IBC R303
Dr Meng-Chiao Ho from IBC “Electron cryotomography 101 & where are we now”
(Symposium) 2020/02/17 “Integrative Structural Biology Workshop” has a cryo-EM related talk “Cryo-EM solutions for structural biology and beyond” from 09:30 to 10:20. More   Poster
(Symposium) 2019/12/27 Conference Hall 1004, Environmental Changes Research Building
2019 Symposium on Statistical Analysis for Molecular Imaging and Biorhythms holds a talk relating to cyro-EM from 14:00 to 14:40. Dr. Wei-Hau Chang “Why we have an atomic-resolution microscope but not always atomic-resolution structures?”
(Seminar) 2019/11/28 Department of Biological Science and Technology, National Chiao Tung University
Dr. Meng-Chiao Ho “CryoEM 101”
(Symposium) 2019/11/12 16:40-17:30 IMB B1 Auditorium
Prof. Wah Chiu from Stanford University “CryoEM for Molecules and Cells” at 2019 IMB Symposium.
(Seminar) 2019/11/06 14:00-15:00 IBC R303
User Meeting: 1. Share ASCEM’s recent development and resources of ECT service 2. Discuss future ASCEM ECT service operation.   Photo1   Photo2
(Seminar) 2019/11/04 Institute of Bioinformatics and Structural Biology, National Tsing Hua University
Dr. Meng-Chiao Ho “CryoEM 101”
(Seminar) 2019/10/26 11:00-12:00 IBC 103 Auditorium
[AS Open House] Dr. Ming-Daw Tsai “Establishment and Progress of Academia Sinica Cryo-EM Center” More
(Symposium) 2019/10/23
“GPU workshop” will focus on CryoEM application. You may directly contact ASGC Dr. Felix Lee felix@twgrid.org Tel: +886-2-27898308 for register. More
(Seminar) 2019/09/27 15:30-16:00 IBC R209
[2019 NTU x ASIBC 9/27 Friday Open House Open for you!] Dr. Meng-Chiao Ho “The Application of Cryo-EM in Structural Biology” (in Chinese) Photo
(Seminar) 2019/09/24 11:00-12:00 IBC R209
Dr. Abhay Kotecha from ThermoFisher Scientific “Micro-Electron Diffraction, Single Particle Analysis and Cryo-Electron Tomography: a hybrid approach for structure determination” Poster   Photo1   Photo2
(Seminar) 2019/08/22 12:00-13:00 IBC R303
1. Dr. Wei-Hau Chang from IoC “Some updates from the past American Microscopy Society Meeting”
2. Prof. Hui-Chih Hung from NCHU “Recent progress in resolving the PAD4-p53-DNA complex structure”
(Seminar) 2019/08/21 15:00 IPMB Auditorium A134, Agricultural Technology Building
Dr. Meng-Chiao Ho “CryoEM 101: Introduction of single-particle cryo-electron microscopy - How Academia Sinica cryoEM center can help you” Poster
(Seminar) 2019/07/25 12:00-13:30 IBC R303
Dr. Gong-Her Wu from Prof. Wah Chiu’s lab at Stanford University "Correlative Cryo-Fluorescence Microscopy and Cryo-FIB/SEM Workflow"
(Seminar) 2019/07/03 12:00-13:00 IBC R303
1. Dr. Erh-Min Lai from IPMB "Biological and mechanistic insights of type IV and type VI secretion systems in Agrobacterium tumefaciens"
2. Dr. Yun Mou from IBMS “Sharing lab experience on the M13 phage particles”
(Symposium) 2019/06/18-2019/06/20 “Workshop on Sample Preparation for Cryo Tomography” Welcome to join the first morning’s talk freely and to register interested hands-on sessions of CLEM or EM ICE without charge. Poster   Photo1   Photo2   Photo3
(Seminar) 2019/06/12 15:30-16:30 GRC 1F Auditorium
Dr. Meng-Chiao Ho “CryoEM101: Introduction of Single-particle Cryo-electron Microscopy – How ASCEM can help you” Poster
(Seminar) 2019/06/05 14:00-16:00 IBC R208
Prof. Jean- François Couture from University of Ottawa “Piecing together a histone methyltransferase complex using structural biology approaches” More
(Seminar) 2019/05/23 12:00-13:00 IBC R303
Dr. Gan-Guang Liou from IBC “The potential tomo project”
(Symposium) 2019/05/29-2019/06/01 “The 24th Biophysics Conference” holds a session of “cryo-EM application” on May 30 13:30-17:00. More
(Symposium) 2019/05/28 9:00-13:30 IBC 103 Auditorium
IBC Biophysics Mini-symposium - from Physics to Diseases holds a talk relating to cryo-EM application from 12:00 to 12:35. Prof. Matthias Wolf “Pilus assembly and Alzheimer's disease - a tale of superhelical reconstruction” More
(Seminar) 2019/05/14 11:00-12:00 talk & 13:30-16:00 ECT101 Q&A IBC R209
Prof. Yi-Wei Chang from Uni. of Pennsylvania “Structural biology in vivo through electron cryotomography”. A Q&A session will be held from 13:30 to 16:00. Welcome to join us freely! Poster   Photo1   Photo2
(Seminar) 2019/04/25 12:00-13:00 IBC R303
User Meeting: 1. Current status of the four microscopes (Dr. Wei-Hau Chang) 2. Application procedure and policy (Dr. Shang-Te Danny Hsu) 3. Scheduling procedure and policy (Dr. Meng-Chiao Joseph Ho) 4. Roles of managers and staff (Dr. Ming-Daw Tsai) 5. Additional issues
(Seminar) 2019/04/11 12:00-13:00 IBC R303
Dr. Cheng-Han Yang from Dr. Ming-Daw Tsai lab "Sharing the preliminary results of model rebuilding on ROSETTA software"
(Seminar) 2019/04/01 11:00-12:30 IBC R209
Mr. Eric Chen from ThermoFisher Scientific. Seeing ultrastructure inside cells in the near-native state! “Electron Cryo-tomography (ECT): FIB”   More   Poster   Photo1   Photo2   Photo3
(Seminar) 2019/03/28 12:00-13:00 IBC R303
Dr. Yu-Chan Chao from IMB “Baculovirus surface display as a possible platform for cryo-EM study of exogenous membrane proteins”
(Symposium) 2019/03/23-2019/03/24 “The 34th Joint Annual Conference of Biomedical Science” holds a session of “Structure Biology / Cryo-EM” on March 24 14:30-16:30. More
(Seminar) 2019/03/14 12:00-13:00 IBC R303
1. Dr. Meng-Chiao Ho “Sharing the current EM results get from ASCEM Titan”
2. Prof. Yoshikazu Takada from UCDAVIS “The integrin-FGF-FGFR ternary complex as a research target for cryoEM”
(Seminar) 2019/02/14 12:00-13:00 IBC R303
1. Dr. Shih-Chi Luo from IBC & Dr. Hung-Yuan Peter Chi from NTU “Recent progress of a helical protein using Titan Krios”
2. Dr. Shang-Te Danny Hsu from IBC “Current EM results got from ASCEM Titan”
(Seminar) 2019/01/31 12:00-13:00 IBC R303
Dr. Po-Huang Liang from IBC “Potential cancer therapy using protein complexation inhibitors”
(Symposium) 2019/01/23-2019/01/25 “The 26th Symposium on Recent Advances in Cellular and Molecular Biology” holds a session of “Novel technology in imaging and single cell analysis / New opportunities in cryo-EM” on January 23 18:30-20:55 and 25 8:00-9:00. More
(Seminar) 2019/01/17 12:00-13:00 IBC R303
1. Dr. Chwan-Deng David Hsiao from IMB “Structure determination of HBV by Cryo-EM".
2. Dr. Chiaho Shih from IBMS will tell us an interesting project.
(Seminar) 2019/01/03 12:00-13:00 IBC R303
1. Dr. Wen-Ching Wang from NTHU will present an interesting project.
2. Dr. Rong-Huay Juang from NTU “Positive charge of Arg-201 on hemagglutinin is required for the binding of H6N1 avian influenza virus to its target through a two-step process”.
(Seminar) 2018/12/20 12:00-13:00 IBC R303
1. Dr. Irene Deli from Dr. Ming-Daw Tsai lab “Sharing experience regarding to the ongoing image processing data got from Titan EM”
2. Dr. Kuen-Phon Wu "1.96 angstrom map of apoferritin"
(Symposium) 2018/12/07-2018/12/11 “2018 Workshop of High Dimensional Statistical Analysis” holds a session of “Probability Theory and its Application in cryo-EM Image Analysis” on December 11. More
(Seminar) 2018/12/06 12:00-13:00 IBC R303
1. Dr. Su-Chang (Tom) Lin “What I learned from a CryoEM sample of about 150kDa”
2. Dr. Gan-Guang Liou "Sharing experiences regarding to the filament EM structures”
(Seminar) 2018/11/22 12:00-13:00 IBC R303
Dr. Gan-Guang Liou "Sharing some EM results regarding to an epigenetic metabolic small molecule, acetyl-ADP-ribose (AAR), relative study."
(Seminar) 2018/11/12 11:00-12:00 ICOB 1F Auditorium
Dr. Sriram Subramaniam “Cryo-EM and potential for use in drug discovery
(Seminar) 2018/11/08 12:00-13:00 IBC R303
Dr. Yoshitaka Bessho & Dr. Wei-Cheng Huang "Structural analysis of mitochondrial-disease related seryl-tRNA synthetase complexed with tRNA"
(Seminar) 2018/10/27 14:00-15:00 IBC 103 Auditorium
[AS Open House] Dr. Meng-Chiao Ho “Application of Cryo-EM on Structural Biology
(Seminar) 2018/10/27 10:45-11:15 GRC 1F Auditorium
[AS Open House] Dr. Su-Chang Lin “The New World-Changing Cryo-Electron Microscopes
(Seminar) 2018/10/25 12:00-13:00 IBC R303
1. Dr. Hung-Jung Wang (from NHRI) "A novel long noncoding RNA, LncR-PKM2, regulates nuclear PKM2-mediated tumorigenesis in prostate cancer"
2. Dr. Peter Hung Yuan Chi (from NTU) "Cryo-EM approach to study recombinase filaments"
(Seminar) 2018/10/11 12:00-13:00 IBC R303
Prof. Li-Mei Pai (CGU) "The structure and biological function of CTP synthase filament"
(Seminar) 2018/09/27 12:00-13:00 IBC R303
1. Prof. Shan-Ho Chou (from NCHU) "Using cryo-EM to study a PKS-NRPS hybrid responsible for a novel anti-fungal factor biosynthesis"
2. Prof. Nien-Jen Hu (from NCHU) "The regulatory roles of signaling nucleotides on Bacillus subtilis KtrA octameric ring"
(Seminar) 2018/09/13 12:00-13:00 IBC R303
Dr. Shu-Yu Huang from Dr. Hsiu-Ming Shih lab “Some preliminary EM results of PML project”
(Symposium) 2018/09/02-2018/09/07 “2018 Short Courses in Frontier Science & Technology - Academia Sinica Cryo-EM Center Grand Opening Symposium & Workshop”, welcome to join us! More
(Seminar) 2018/08/30 12:00-13:00 IBC R303
1. Dr. I-Ping Tu “Developing a cryo-EM computation platform with focus on integration and visualization”
2. Dr. Shang-Te Danny Hsu will present a cryoEM progress of his project.
(Seminar) 2018/08/16 12:00-13:00 IBC R303
Dr. Joseph Meng-Chiao Ho “Sharing interested results gotten from our Talos artica EM recently”
(Seminar) 2018/08/02 12:00-13:00 IBC R303
Dr. Chin-Yu Chen “Cryo-EM structure of the thermoactive ketol-acid reductoisomerase (KARI) from Sulfolobus solfataricus”
(Seminar) 2018/07/19 12:00-13:00 IBC R303
1. Dr. Wei-Hau Chang lab “Some of current results got from our Talos recently”
2. Dr. Kao from Dr. Hsien-Ming Lee lab “Re-investigate membrane lytic peptide with covalent proximity: peptide-membrane interaction mechanism study and liposome trigger release application”
(Seminar) 2018/07/05 12:00-13:00 IBC R303
Dr. S.-J. Su from Dr. Han-Chung Wu lab “Presenting his project regarding to Dengue virus on F20 cryo-EM”
(Seminar) 2018/06/21 12:00-13:00 IBC R303
1. Tzu-Jing Yang from Dr. Shang-Te Danny Hsu lab “Presenting his interested project regarding to a feline (cat) corona virus”
2. Dr. Chia-Lung Li form Hanna S. Yuan lab “EM studies on human mitochondrial PNPase and Suv3 in RNA turnover”
3. Dr. Su from Dr. Han-Chung Wu lab “Presenting his project (on F20) on Dengue virus”
(Seminar) 2018/05/24 12:00-13:00 IBC R303
Dr. Kuen-Phon Wu “Shareing what he gets/learns from our new Talos artica EM recently”
(Symposium) 2018/05/23-2018/05/25 “The 23rd Biophysics Conference” holds a session of “Cryo-EM Application” on May 23 14:40-17:10. Welcome to join us! More
(Seminar) 2018/05/10 12:00-13:00 IBC R303
Dr. Wei-Hau Chang “Sharing what his lab get/learn from our new Talos artica EM recently”
(Seminar) 2018/04/26 12:00-13:00 IBC R303
Dr. Piotr Draczkowski “The story about the proteasome”
(Seminar) 2018/03/29 12:00-13:00, IBC R303
Dr. Su-Chang Lin (林世昌助研究員) “Sharing his harvests from the cryo-EM course held in Oregon Health & Science University from February 5 to March 2, 2018”
(Seminar) 2018/03/20 11:00-12:00 IBC R114
Dr. Matthias Wolf “The power and beauty of single particle cryo-EM
(Seminar) 2018/03/15 12:00-13:00, IBC R303
Dr. Meng-Chiao Ho (何孟樵助研究員) “Sharing his harvests from the cryo-EM course held in Oregon Health & Science University from February 5 to March 2, 2018”
(Seminar) 2018/03/06 11:00-12:00 IBC R114
Dr. Kuen-Phon Wu “Structural insight into activation of HECT E3 ligase: implications to human diseases
(Seminar) 2018/02/27 11:00-12:00 IBC R114
Dr. Pei-Chun Lin “CryoEM studies of pre-mRNA splicing reveal assembly and activation of the spliceosome
(Seminar) 2018/01/04 12:00-13:00, IBC R303
Dr. Kuen-Phon Wu "Sharing his successful GraFix experimental experience"
(Seminar) 2017/11/22 12:00-13:00, IBC R303
Dr. Yi-Min Sean Wu “Sharing his harvests at New York - Part II”
 
 
Other sources for self-study
 
  Caltech cryoEM courses:
      Grant Jensen cryoEM lectures (49 videos)
      Cryo-EM course - Unit 2: Sample Preparation (42 videos)
  iBiology channel
      Introduction to electron microscopy by Eva Nogales
      Single Particle CryoEM by Yifan Cheng
  NYSBC NRAMM SEMC channel (~30 videos, 1-4 hours per video)
  MRC-LMB
      MRC-LMB 2014 Electron cryo-microscopy course (10 videos)
      MRC-LMB 2017 Electron cryo-microscopy course (10 videos)
  Nobel Lectures
      Richard Henderson
      Joachim Frank
      Jacques Dubochet
      (Or full video for 3 lecture in Chemistry)
  SBGrid Consortium @ Youtube
      EMAN2.2
      SPHIRE
      RELION
      ChimeraX
      xMDFF
 

Contact: Ms. Hsin-Hua Lin, Academia Sinica CryoEM Center   Email:cryoem@gate.sinica.edu.tw

No. 128, Sect. 2, Academia Rd., Nankang, Taipei 115, Taiwan (R.O.C.)      TEL:(02) 27855696 #3020

(Lab) B2-I04 (Interdisciplinary Sci. & Tech. Building), No. 28, Ln. 70, Sec. 2, Academia Rd., Nankang, Taipei 115, Taiwan (R.O.C.)

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